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Greenspring Tm Clenbuterol Elisa Test Kit
Posted at: Offers to Sell and Export | Posted on: Tuesday 8 December 2009 1:45 am | Poster last visit: Thursday 4 December 2014 |
Product Description:
Sensitivity: 0.1 ppb
Detection limit
Tissue 0.1 ppb
Urine, serum 0.1 ppb
Feed 10 ppb
Recovery
rate
Urine, serum 90±10%
Tissue 80±10%
Feed 80±15%
Cross-reaction rate
Clenbuterol 100%
Mabutero 95%
Brombuterol 115%
Salbutamal < 11%
Terbutalin < 7%
Ractopamine < 1%
ELISA procedures
1. Bring test kit to the room temperature (20-25 ℃) for at least 30 min, note that each reagent must be shaken evenly before use; put the required micro-well strips into plate frames. Re-sealed the unused microplate, stored at 2-8 ℃, not frozen.
2. Numbering: number the micro-wells according to samples and standard solution; each sample and standard solution should be performed in duplicate; record their positions.
3. Add 20 µL of the sample or the standard solution into separate duplicate wells, then add enzyme conjugate, 50 µL/well; and antibody working solution, 80 µL/well. Mix gently by shaking the plate manually, seal the microplate with the cover membrane, incubate at 25 ℃ for 30 min.
4. Pour liquid out of microwell, flap to dry on absorbent paper; add 250 µL/well of tri-deionized water, wash for 4-5 times, 15-30 s each time, then take out and flap to dry with absorbent paper.
5. Coloration: add 50 µL of substrate A solution and 50 µL B solution into each well. Mix gently by shaking the plate manually, and incubate at 25 ℃ for 15 min in the dark for coloration.
6. Determination: add 50 µL of the stop solution into each well. Mix gently by shaking the plate manually. Set the wavelength of the microplate reader at 450 nm to determine the OD value of every well. (Recommend to read the OD value at the dual-wavelength 450/630 nm within 5 min).
Company Contact:
Contact Name: Jiang Yongqing
Company Name: Shenzhen Lvshiyuan Biotechnology Co, Ltd
Email:
Tel: +86-755-21567288
Fax: +86-755-28938800
Street Address: Rm.507, No.2,
Longgang Overseas Venture Park,
Shenzhen, China
Website: https://www.lsybt.com
Member name: Shenzhenlvshiyuanbiotec
Country:
China
Member Since: 07 March 2008
Total Leads: 69 Shenzhenlvshiyuanbiotec Import Export Business Leads
Business focus: Animal Disease Test Kit, Food Safety Test Kit
Chat:
Verify: Safe Import Export Tips
Detection limit
Tissue 0.1 ppb
Urine, serum 0.1 ppb
Feed 10 ppb
Recovery
rate
Urine, serum 90±10%
Tissue 80±10%
Feed 80±15%
Cross-reaction rate
Clenbuterol 100%
Mabutero 95%
Brombuterol 115%
Salbutamal < 11%
Terbutalin < 7%
Ractopamine < 1%
ELISA procedures
1. Bring test kit to the room temperature (20-25 ℃) for at least 30 min, note that each reagent must be shaken evenly before use; put the required micro-well strips into plate frames. Re-sealed the unused microplate, stored at 2-8 ℃, not frozen.
2. Numbering: number the micro-wells according to samples and standard solution; each sample and standard solution should be performed in duplicate; record their positions.
3. Add 20 µL of the sample or the standard solution into separate duplicate wells, then add enzyme conjugate, 50 µL/well; and antibody working solution, 80 µL/well. Mix gently by shaking the plate manually, seal the microplate with the cover membrane, incubate at 25 ℃ for 30 min.
4. Pour liquid out of microwell, flap to dry on absorbent paper; add 250 µL/well of tri-deionized water, wash for 4-5 times, 15-30 s each time, then take out and flap to dry with absorbent paper.
5. Coloration: add 50 µL of substrate A solution and 50 µL B solution into each well. Mix gently by shaking the plate manually, and incubate at 25 ℃ for 15 min in the dark for coloration.
6. Determination: add 50 µL of the stop solution into each well. Mix gently by shaking the plate manually. Set the wavelength of the microplate reader at 450 nm to determine the OD value of every well. (Recommend to read the OD value at the dual-wavelength 450/630 nm within 5 min).
Company Contact:
Contact Name: Jiang Yongqing
Company Name: Shenzhen Lvshiyuan Biotechnology Co, Ltd
Email:
Tel: +86-755-21567288
Fax: +86-755-28938800
Street Address: Rm.507, No.2,
Longgang Overseas Venture Park,
Shenzhen, China
Website: https://www.lsybt.com
Member name: Shenzhenlvshiyuanbiotec
Country:
ChinaMember Since: 07 March 2008
Total Leads: 69 Shenzhenlvshiyuanbiotec Import Export Business Leads
Business focus: Animal Disease Test Kit, Food Safety Test Kit
Chat:
Verify: Safe Import Export Tips
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