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Recombinant Human Abcb1
Posted at: Offers to Sell and Export | Posted on: Friday 14 September 2012 3:49 am | Poster last visit: Monday 8 October 2012 |
Detection of ATPase activity of the MDR1 protein is a measure of transporter
activity. The assay is performed using purified membrane vesicles from Sf9 (Spodoptera frugiperda) cells, expressing high levels of MDR1 protein. The ABC transporters pump substrates out of the cell by using hydrolysis of ATP as an energy source. ATP hydrolysis yields inorganic phosphate (Pi), which can be detected by a simple colorimetric reaction. The amount of Pi liberated is proportional to the activity of the transporter.
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